Heterologous expression of rat epitope-tagged histamine H2 receptors in insect Sf9 cells
نویسندگان
چکیده
منابع مشابه
Heterologous expression of rat epitope-tagged histamine H2 receptors in insect Sf9 cells.
1. Rat histamine H2 receptors were epitope-tagged with six histidine residues at the C-terminus to allow immunological detection of the receptor. Recombinant baculoviruses containing the epitope-tagged H2 receptor were prepared and were used to infect insect Sf9 cells. 2. The His-tagged H2 receptors expressed in insect Sf9 cells showed typical H2 receptor characteristics as determined with [125...
متن کاملVectors for inducible expression of dual epitope-tagged proteins in insect cells.
Expression and purification of recombinant proteins are fundamental techniques in molecular biology. Many different cell systems ranging from bacteria to mammalian cell lines are commonly used. One of the most widely used systems is the baculovirus expression system. An alternative for large-scale production of eukaryotic proteins are stably transfected Schneider Drosophila line 2 cells (Catalo...
متن کاملProtocol for Heterologous Expression of Insect Odourant Receptors in Drosophila
Insect olfactory receptors (ORs) are tuned to volatile chemicals, they are expressed in the membrane of olfactory sensory neurons (OSNs), housed in sensilla on the antenna. The olfactory apparatus is under strong selection and ORs are tuned to vital chemical signals, mediating social communication, feeding and oviposition, and avoidance of predators and pathogens. An emerging technique to relia...
متن کاملHistamine modulates contraction and cyclic nucleotides in cultured rat mesangial cells. Differential effects mediated by histamine H1 and H2 receptors.
Histamine influences the glomerular microcirculation and modulates immune-inflammatory responses. In the rat kidney, histamine is synthesized by glomeruli and stimulates cyclic nucleotide production specifically in glomeruli. We investigated the in vitro effect of histamine on cyclic nucleotide accumulation in rat cultured glomerular mesangial and epithelial cells. Histamine stimulated cyclic A...
متن کاملHistamine suppresses gene expression and synthesis of tumor necrosis factor alpha via histamine H2 receptors
Histamine and tumor necrosis factor alpha (TNF-alpha) can each contribute to the pathogenesis of allergic reactions and chronic inflammatory diseases. We now report the effect of histamine on gene expression and total cellular synthesis of TNF-alpha. Lipopolysaccharide (LPS)-induced synthesis of TNF-alpha in peripheral blood mononuclear cells (PBMC) from 18 healthy donors was suppressed by hist...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: British Journal of Pharmacology
سال: 1997
ISSN: 0007-1188
DOI: 10.1038/sj.bjp.0701466